Dayoung Park, Daesup Geum, Young Ji, Yong-Nam Pak
It is very important to monitor the concentration of selenium in the body precisely because of its narrow range of necessity and toxicity. In order to obtain biologically meaningful information, Se speciation study must be carried out for its bio-activities. Human nail samples, as well as hair are useful biological samples that reflect long-term selenium exposure while breath can reflect for a relatively short-term.. Researches have been carried to find out the optimum extraction condition and accurate determination of Se species with Isotope Dilution HPLC-ICP-MS. Nails were treated with NaOH solution at 90 °C for 24 hours, the degradation efficiency was about 70%. Selenium species were separated using Anion exchange HPLC to detect SeCys and Se4+. The concentration of selenium species was analyzed SeCys species was shown in the range of 700-950 ppb for a year. When a selenium supplement was given (300 μg day-1), it was found out that the selenium in nails increased within 2 months and depleted after while a regular supplement given (22 μg day-1) did not show much effect. Hair samples showed around 200 ppb, higher values at root area. Breath samples were also analyzed and only a trace amount of DMSe was shown. This research shows that human samples such as hair, nails and even breath can be used to monitor Se species in our body effectively either in a long or short period of exposures.
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